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Histopathology

Immunohistochemistry Staining Methods

Identify Specific Proteins, Cells or Structures

Immunohistochemistry and Immunofluorescent

Staining Methods

Immunohistochemistry (IHC) is a highly useful technique for detection and quantification of target epitopes (e.g proteins, structures, cellular components, etc) in a wide variety tissue types. Applicable to fresh, frozen and FFPE samples, IHC utilizes high specificity antibodies for visualization of target expression patterns, providing quantitative, qualitative and spatiotemporal information on processes taking place in tissues. Antibody-epitope interactions are visualized through secondary staining of samples with an additional antibody that has been conjugated to either a color-producing enzyme (chromogenic staining) or a fluorophore (immunofluorescent staining, or IF).  


 

Immunofluorescent Staining 

 Immunofluorescent staining (IF) detects specific markers in tissue sections using primary antibodies to specifically bind to target antigens, which can then be visualized utilizing various fluorescent tags. This technique adds the value of multiplexing, where multiple targets can be localized within a single cell.

6x-magnification

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TregTh17 Panel 1 website snapshot

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Advanced capabilities for antibody validation & staining optimization

Contact us regarding your specific needs in assay development and antibody optimization.

 Available Species:

  • Mouse
  • Rat
  • Human
  • Porcine
  • Primate

Experience with non-standard tissue types

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