Immunohistochemistry (IHC) is a highly useful technique for detection and quantification of target epitopes (e.g proteins, structures, cellular components, etc) in a wide variety tissue types. Applicable to fresh, frozen and FFPE samples, IHC utilizes high specificity antibodies for visualization of target expression patterns, providing quantitative, qualitative and spatiotemporal information on processes taking place in tissues. Antibody-epitope interactions are visualized through secondary staining of samples with an additional antibody that has been conjugated to either a color-producing enzyme (chromogenic staining) or a fluorophore (immunofluorescent staining, or IF).
Immunofluorescent staining (IF) detects specific markers in tissue sections using primary antibodies to specifically bind to target antigens, which can then be visualized utilizing various fluorescent tags. This technique adds the value of multiplexing, where multiple targets can be localized within a single cell.
Advanced capabilities for antibody validation & staining optimization
Contact us regarding your specific needs in assay development and antibody optimization.